YAP manipulates proliferation via PTEN/AKT/mTOR-mediated autophagy in lung adenocarcinomas

Abstract Background Autophagy is a double-edged sword during the initiation and progression of multiple tumors. The Hippo pathway effector YAP has been proved to be involved in autophagy processes. present study aimed investigate how regulates cell proliferation via lung adenocarcinomas (LUAD). Methods Data LUAD chip GSE43458 was obtained from Gene Expression Omnibus (GEO). RT-qPCR Western blot were performed assess expression lines. CCK-8 assay, xenograft tumor model, immunochemistry GFP-mRFP-LC3 fusion proteins utilized evaluate effect on cells vitro vivo . inhibitor treatment rescue experiments carried out elucidate mechanism by which manipulates cells. Results significantly overexpressed samples patients its level related 5-year survival. manipulated A549 H1299 could induce activation Akt/mTOR signaling suppressing PTEN Hippo-pathway-dependent manner. 3-Methyladenine impeded flux promoted Conclusions critical transcriptional coactivators adenocarcinoma, regulated PTEN/AKT/mTOR autophagic signaling.